Cryopreservation of dental tissue and subsequent isolation of mesenchymal stem cells
نویسنده
چکیده
inhibiting T-helper cells while activating regulatory T-cells and suppressing B-lymphocytes. This immunomodulatory effect of MSCs makes them suitable for treatment of severe autoimmune diseases and graft-versus-host disease. However, most trials for the clinical application of MSCs have used bone marrow-derived MSCs (BMSCs). As mentioned previously, MSCs from dental tissue (DMSCs) exhibit similar characteristics to BMSCs, but have superior osteogenic differentiation potential. Therefore, DMSCs could possibly replace BMSCs in cell therapy and tissue regeneration applications. The new cryopreservation method for dental tissue enables the use of autologous MSCs from preserved dental tissue of extracted wisdom teeth. These autologous MSCs could reduce unexpected side effects during clinical use, while maintaining similar immunomodulatory efficacy. In conclusion, dental tissue from immature wisdom teeth, including that from the dental follicle, pulp, and root apical papilla, possesses abundant multipotent stem cells that could be useful in both cell therapy and tissue regeneration. Traditionally, these dental tissues have been discarded after extraction. In the future, dental tissue obtained via extraction of impacted teeth should be cryopreserved for use as an autologous stem cell source. The long-term preservation of dental tissue after tooth extraction could thus create a new business model in dentistry, as well as provide greater access to autologous MSCs for patients in need.
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